Complementary Assays to Evaluate B Cell Tumor Killing Efficiency using CD19-BiTEs on Cellular and Molecular Level

A new application note describes the coupling of quantitative cell assays with biomarker quantitation to assess killing of B cells by CTLs (cytotoxic T lymphocytes).

The workflow comprises the use of xCELLigence RTCA Cytotoxicity Assays for kinetic assessment of the killing process,  as well as confirmation experiments using flow cytometry-based Cytolysis Assays. Finally, molecular phenomena are integrated by quantitative and bead-based multiplex immunoassays of cytokine and effector proteins.  

It is remarkable that both, cell samples and cell media were removed from xCELLigence experiments for confirmation experiments using flow cytometry and for cytokine and effector molecules analysis - due to the fact that cells are not destroyed by xCELLigence RTCA assays and thus are available for complementary analysis.

Read Application Note

 → also available: CYTO 2017 Workshop Recording



Passende Artikel

xCELLigence MP

Das hochflexible System für hohen Datendurchsatz oder Multi-User Einsatz xCELLigence MP: Multi-Plate System - für 6 x 96 well E-Plates. Hochflexibel: für den hohen Datendurchsatz oder parallele Experimente mehrerer Anwender. Dynamische markerfreie Echtzeit Zell-Analytik mit hoher Datenqualität, weitem Anwendungsbereich, vollständig automatischer Daten-Aufzeichnung und vielseitiger intuitiver Analysesoftware. Die...

Preis auf Anfrage


NovoCyte Flow Cytometer

The new NovoCyte. Your personal declaration of independence in flow cytometry. Available there where you need it : in your lab. It grows with your needs : Expandable at any time. Powerful :Best in Class at an amazing price-performance ratio. Intuitive :Load & Go software Get the manifold possibilities of a Flow Cytometer in your lab A flow cytometer provides virtually unlimited...

Preis auf Anfrage



Kommentar schreiben


Die mit einem * markierten Felder sind Pflichtfelder.