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Continuous, marker-free Cell Analysis: the xCELLigence Systems

Exceed limitations from Endpoint-Assays
Non-invasive, label-free, full kinetic information real-time cell analysis: The ACEA RTCA systems analyze your cells on-line while adhered in culture. The analyzers are easy to operate and do not require any label, marker, reporter or dye. Measuring the impedance with utmost precision provides a wealth of information on all processes going on inside the cells. Readings are taken in programmable intervals from 30 sec minimum onwards and thus provide information an end-point assay can never provide.

Key Applications

  • Adhesion and Proliferation assays
  • Receptor-Interaction assays
  • Immuno-Oncology: Screening tumor cell killing
  • Cytotoxicity assay (compound-, virus-, cell- and antibody induced)
  • Cardio toxicity and Arrhythmia assays (xCELLigence Cardio only)
  • Cell-to-cell interaction assays (direct contact or by means of the E-Plate Co-culture inserts)
  • Continuous quality control of media, media components and cells
  • Cell migration assays (Migration and invasion assays, only system xCELLigence DP) replace e.g. Boyden chamber or comparable assays

Key Features

  • Marker-free, no reporter substances, no overexpression: No external interference, real physiological conditions
  • Dynamic real-time monitoring: Provides complete kinetic information to validate/optimize end-point assays
  • Non-invasive technology: Run long-term experiments up to several 100 hours under exact physiological conditions

Can do much more than an Endpoint-Assay

Exceed a conventional endpoint assay significantly by continuous monitoring of cell interactions and understand cellular responses much better: Application Example: → "Virus-mediated Cytopathogenicity"

 

Video: Monitoring of the invasion behavior of cancer cells in real-time 


Metastatic spread of malignant tumors by invasion of the endothelium and finally into the blood circulation can be shown in vitro e.g. by the invasion of HUVEC cell monolayers. The RTCA impedance measurement with xCELLigence allows online monitoring of this process - without any influence by dye additions or the like.

A short 9-minute video summarizes the establishment and implementation of such assays: xCELLigence RTCA technology outperforms the classical Boyden Chamber Matrigel assays twice:

  • The procedure is close to the in vivo conditions 
  • The data is available in real time before and can be easily quantified

Video: A Real-time Electrical Impedance Based Technique to Measure Invasion of Endothelial Cell Monolayer by Cancer Cells; JOVE - Journal of Visualized Experiments doi: 10.3791/2792

 

 

Cytotoxicity, Migrations-Assay and Cell-Adhesion - have a look "live in the lab":

Watch three short video clips and learn about the workflow of a cytotoxicity assay and a cell migration assay with xCELLigence systems in the laboratory. Based on the iCELLigence system, we also present a cell adhesion assay: Learn how to do cell adhesion assays with the handy, iPad-controlled iCELLigence real time cell analyzer.

watch a french version of the video here: https://youtu.be/CVCSqYO0hCY

 

 

Application areas xCELLigence: Find out more.

IMMUNO-ONCOLOGY - xCELLigence monitors killing activity online.

→ read more.

 

CELL MIGRATION & INVASION - comprehensive kinetics for Chemotaxis-Assays

→ read more

 

 

Titel / BeschreibungBestellnummerMengePreis
iCELLigence

iPad-controlled system – for 1 or 2 8well plates. Adhesion, proliferation, cytotoxicity, receptor activity, barrier function, 64 square mm per well

Preis auf Anfrage

2801015

xCELLigence DP

Dual Purpose System – additionally: Migration/Invasion assays (CIM); offers space for three E- or CIM-plates which can be used independently or freely combined

Preis auf Anfrage

2801006

xCELLigence SP

Single Plate System – for Real-Time Cell Analysis in 96-well format with minimum space consumption in the incubator.

Preis auf Anfrage

2801000

xCELLigence MP

Multi Plate System – for 6 x 96-well E-Plates. Enables up to six 96-well experiment run in parallel and independently.

Preis auf Anfrage

2801003

xCELLigence HT

High Throughput 384-well system, to be integrated in automation platforms. Upgradeable up to 4 work stations à 384 wells.

Preis auf Anfrage

2801012

HT Workstation

Additional work station for the xCELLigence HT system, incl. Heating and Temperature control.

Preis auf Anfrage

2801025

xCELLigence CARDIO

The reference system for cardiotoxicity and arrhythmia assays. Real-time Monitoring of cardiomyocyte contractility.

Preis auf Anfrage

2801009

xCELLigence CardioECR

The reference system for cardiotoxicity, arrhythmia and ion channel field potential assays. Real-time Monitoring of cardiomyocyte contractility.

Preis auf Anfrage

2801190

   
 
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E-Mail: britta.gerlach@ols-bio.ch
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