xCELLigence HT

Hersteller ACEA Biosciences Inc.

Artikel-Nr.: 2801012

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xCELLigence HT

The Superior Technology to get a Wealth of Information on the Biological Status of Your Cells
xCELLigence HT:
High-throughput system based on 384-well format E-Plates. The HT system integrates well with automated sample preparation robots from many manufacturers and joins the RTCA technology for live cells into the high-throughput screening process. One analyzer can link with up to 4 work stations each holding one 384-well E-Plate resulting in a maximum capacity of 1536 samples. The work station dimensions are designed to fit into most robot systems. The very flexible system easily adapts to real time high throughput cell analysis or independent experiments of several users. Run your Real Time Cell Analysis (RTCA) fully automated and label-free to produce data of highest quality and integrity. The software covers a broad application range and incorporates a very comprehensive and flexible analysis tool. Get a wealth of information on the biological status of your cell cultures.

Key Applications

  • High Throughput assays, integrates into HTS robots of many manufacturers (e.g. Hamilton, Beckman, Agilent)
  • Classical cell-based assays (proliferation, adhesion, viability, morphology etc.)


High-Troughput Assays, integrates in many robot platforms
The xCELLigence HT system is a high throughput analyzer for the label-free real time cell analysis in 384-well plate format. Up to 4 work stations can be connected to the analyzer to yield a total of 1536 samples per run, if desired. The compact design work station is integrated into the automation platform (with or w/o incubator access) whereas experiment control and data evaluation are handled by the external analyzer and a PC (laptop). All active assays are monitored continuously. 

  • Integrates easily into high-throughput robot systems
  • Very high throughput of up to 4x 384 wells = 1536 samples per run 
  • User administration enables up to 4 independent and simultaneous analyses 
  • Continuous recording of cells response signals in all cell based assays
  • Optimizes timing for compound application and/or downstream assays
  • Excellent reproducibility and data integrity (<0.3% well failures, <15% intra/inter plate variation)
  • Fully automated data acquisition throughout the whole experiment
  • Long term experiments under controlled physiological conditions in the incubator
  • Compact design of work station, needs only very small space in incubator
  • Easy to handle, intuitive user interface for programming the test protocol
  • Data Analysis software integrated

Method of Operation
Cells are seeded and grown on an E-plate equipped with gold-plated electrodes. The changes in morphology of the adhered cells cause changes in impedance of the electrodes. This allows to monitor all changes in cell status continuously and dynamically. As data are recorded continuously, this method of real time monitoring produces a wealth of information far superior to classical end-point assays. This technology is currently making its way into drug development, toxicology, cancer research, microbiology and virology.


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