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Ecotoxicology Testing using CASY

Phytotoxic effects of chemical pollutants are often performed using cell growth assays with unicellular green algae. In this study, the toxicity of the antibiotic ciprofloxacin to the green algae Raphidocelis subcapitata was investigated using CASY.

 

The cell cycle of R. subcapitata consists of three phases: growth of the mother cell, cell division
(which includes two divisions of the nucleus), and the release of four autospores. Over a period of 24h, the cell number and size distribution of a synchronized culture was tracked, revealing phases of cell growth and phases of proliferation.