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The destruction of Daudi B cells by T cells in the presence or absence of a Bispecific T Cell Engager (BiTE) was analyzed in real-time using the xCELLigence technology. Concurrently, because cells are not destroyed by this technique, it was possible to remove samples at select time points and confirm cell killing by flow cytometry. Moreover, by removing media from select wells of the xCELLigence microtiter plate, the secretion of 7 different cytokines and 6 different effector molecules were also analyzed using a bead-based multiplex assay combined with flow cytometry.