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Register now for our webinar "Sensitive Yet Realistic Assessment of CAR T Cell Killing Capacity Against Solid Tumors"

 

Killing Cancer Cells: Pictures start Moving

How to transform your Cytotoxicity Assays into a thriller: Look beyond the endpoint-assay. This article compares current technologies.

 

Prior to in vivo studies, by using the xCELLigence platform for a read-out over 30hours, it has been shown that epigenetic upregulation of NY-ESO-1 by DAC sensitize 2 glioblastoma cell lines to NY-ESO-1–T cells killing, at the physiological ratio of 1:1.

 

A recent publication by A J Davenport et al in Cancer Immunology Research demonstrates the "serial killing" nature of individual CAR cells by killing multiple tumor cells. Killing assays were performed using xCELLigence.

 

Main advantages using xCELLigence killing assays: It reveals kinetic differences that cannot be captured with fixed end point platforms, it uses a minimum of cells, saves time and correlates well with standard cytotoxicity measurements.

 

CAR-T Discovery: Utilizing Real-Time Cell Analysis Technology
4.11.2015, 17:00 Europe Time (Berlin, GMT +01:00)

 

ACEA's xCELLigence technology offers unique advantages in assesing cell- and antibody-dependent cytotoxicity, since it's label-free and kinetic read-out allows for medium to high throughput screening under physiological conditions. Numerous peer-reviewed publications have demonstrated the usefulness of the xCELLigence instruments for the assessment of immune-mediated tumor cell killing

 

Based on electrical impedance measurements (using the xCELLigence system), the authors established a label-free assay, facilitating the real-time monitoring of T-cell-mediated cytotoxicity. The coculture of peptide-specific T-cell lines with peptide-loaded target cells reproducibly led to a decrease in impedance due to induced apoptosis and detachment of target cells. Comparing our results to the standard CRA assay, we could demonstrate that impedance-based measurements show comparable results after short incubation periods (6 h) but outperform the CRA both in reproducibility and sensitivity after prolonged incubation (24 h), enabling the detection of target cell lysis with an effector to target ratio as low as 0.05:1.