We combine xCELLigence assays with NovoCyte ?ow cytometric analyses to characterize two key immunological prophylactic mechanisms. First, we track Bispeci?c T Cell Engager (BiTE)-mediated stimulation of cancer cell killing by T cells. Next, we shift our focus to NETosis, a cell death process that occurs when stimulated neutrophils cast an extracellular web containing DNA, histones, and anti-bacterial proteins that ensnare/neutralize invading organisms.
The destruction of Daudi B cells by T cells in the presence or absence of a Bispecific T Cell Engager (BiTE) was analyzed in real-time using the xCELLigence technology. Concurrently, because cells are not destroyed by this technique, it was possible to remove samples at select time points and confirm cell killing by flow cytometry. Moreover, by removing media from select wells of the xCELLigence microtiter plate, the secretion of 7 different cytokines and 6 different effector molecules were also analyzed using a bead-based multiplex assay combined with flow cytometry.
November 17: We will present the importance of studying free radicals, their dual roles as both toxic and beneficial compounds in cellular response and immune function. We will also introduce a new panel of multicolor CytoTell™ cell proliferation and tracking dyes that are well excited by major NovoCyte laser lines.
