What's new? This blog keeps you up-to-date with the latest news, events and campaigns at OLS.

Ecotoxicology Testing using CASY

Phytotoxic effects of chemical pollutants are often performed using cell growth assays with unicellular green algae. In this study, the toxicity of the antibiotic ciprofloxacin to the green algae Raphidocelis subcapitata was investigated using CASY.

 

Based on electrical impedance measurements (using the xCELLigence system), the authors established a label-free assay, facilitating the real-time monitoring of T-cell-mediated cytotoxicity. The coculture of peptide-specific T-cell lines with peptide-loaded target cells reproducibly led to a decrease in impedance due to induced apoptosis and detachment of target cells. Comparing our results to the standard CRA assay, we could demonstrate that impedance-based measurements show comparable results after short incubation periods (6 h) but outperform the CRA both in reproducibility and sensitivity after prolonged incubation (24 h), enabling the detection of target cell lysis with an effector to target ratio as low as 0.05:1.

 

Webinar "Toxicity Bioassays - Unleashing the Power of Real-Time Cell Analysis" steht nun zum Abruf bereit