Growth Dynamics of Biofilm Formation Monitored using xCELLigence

Growth Dynamics of Biofilm Formation Monitored using xCELLigence

A new nature publication describes the benefits using xCELLigence continuous label-free measurements to characterize biofilm formation and its modulation by small molecule drugs. Published as an open scientific report on (doi:10.1038/s41598-017-05273-5), the authors followed impedance spectroscopy of P. aeruginosa over a time period of 72 hours.

Distinct phases of biofilm formation were revealed by continuous measurements. The disclosure of even subtle perturbances of a biofilm was enabled by a higher dynamic range and a smaller standard deviation of xCELLigence impedance assays as compared to standard crystal violet staining.

Monitoring the effects of antibiotics using high precision impedance assays, the authors showed impaired biofilm formation as a consequence of higher antibiotics concentrations, while lower concentrations also were shown to alter impedance in a substance-specific manner, but did not impair bacterial growth.

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Figure: Typical curve shapes of biofilm formation

Changes in the standardized single-frequency impedance (expressed as cell indices) of pellicle biofilm over 72 h, during biofilm growth in LB culture medium of PA14 wt, pqsA::Tn mutant, and pelA::Tn mutant (n = 24 for each mutant) and LB Medium (n = 8). The PA14 wild type and pqsA::Tn are biofilm formers; pelA::Tn does not form biofilm. n indicates the number of technical replicates. The period of linear decline of the cell index is highlighted by a yellow bar.

from: Use of Single-Frequency Impedance Spectroscopy to Characterize the Growth Dynamics of Biofilm Formation in Pseudomonas aeruginosa. Jozef B. J. H. van Duuren, Mathias Müsken, Bianka Karge, Jürgen Tomasch, Christoph Wittmann, Susanne Häussler & Mark Brönstrup. Scientific Reports 7, Article number: 5223 (2017) doi:10.1038/s41598-017-05273-5



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