ADCC of adherent target cells: How to improve prediction of treatment efficacy

In a new paper (Anabel Zwick, et al., Journal of Immunological Methods, https://doi.org/10.1016/j.jim.2019.112644), the autors report results from granulocyte-mediated ADCC obtained using real-time monitoring with xCELLigence (ACEA Biosciences) .

While the impedance assay overcame limitations of end-point assays, such as the requirement of target cells in suspension or unspecific release of probes, results at short-term were comparable to 51-chromium or Calcein release assays. 

However, in the long run, real-time monitoring revealed additionally cell-line specific kinetics and long-term efficacy. Monitoring IgA-dependent neutrophil-mediated ADCC for more than four hours allowed the documentation of residual non-killed target cells that grew out again, although short-term kinetics indicated efficient killing.

The authors discuss additional critical parameters for the prediction of treatment efficacy appearing only after prolonged monitoring of ADCC.

In conclusion, xCELLigence provides additional valuable information on kinetics of ADCC against target cells, effector cell performance, efficacy and additional critical parameters of cytotoxicity.

 

 

 
 

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