NovoCyte Advanteon Flow Cytometer

 

Expanded Capabilities, Simple Operation.

The new NovoCyte Advanteon flow cytometer builds upon the highly successful NovoCyte, and NovoCyte Quanteon, to provide an advanced set of capabilities for the most demanding scientists, yet is remarkably simple to operate.

 Advanteon accommodates today’s high-end multi-color flow cytometry assays, and provides the flexibility of 1, 2, or 3 laser options, with up to 21 fluorescence channels and 23 independent detectors.

 Customize the instrument to meet your specific needs, but easily upgrade to meet your future demands.

 

Automation-ready

When throughput is essential, the NovoSampler Q can be integrated into different laboratory automation platforms, and can efficiently process both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates.

 For the NovoCyte Advanteon, the intuitive and industry honored NovoExpress® software is now even more advanced, providing an exceptional user experience in data acquisition, analysis, and reporting.

  

  • Expanded flexibility with up to 21-color options
  • 1-3 lasers
  • customizable and upgradeable
  • Sample recovery mode (collect unused sample at end of acquisition)
  • Superior sensitivity and resolution
  • Intuitive and powerful software (data acquisition, analysis, and reporting)
  • Walk-away operation
  • Automation-ready for high throughput needs
  • Wide, 7.2-log dynamic range
  • High speed collection up to 100,000 events/second
  • Accurate absolute cell count in every experiment

 

 

Flexibility in Panel Design

 With up to 21 fluorescence channels, Advanteon offers tremendous flexibility in panel design, thereby allowing more data to be collected per run. Assessment of T cell state and function is just one critical example for researching cell-based immunotherapies using multi-color staining panels.

 

Analysis of T cell differentiation using the NovoCyte Advanteon: Monitor T cell exhaustion and measure cytokine proliferation from PBMC’s following activation, all in one experiment.

 

The Ultimate Photodetector

Silicon photomultipliers (SiPM) are solid-state, photon-level sensitive semiconductor devices. With a compact array of avalanche photodiodes operating in unison, the SiPM found in the Advanteon can provide photon counting capability. The innovative optical design incorporates 21 independent SiPM, which collects light on each and every channel, with sensitivities that far exceed the traditional PMT’s.

 

SiPM’s Strengths

  • Superior photon detection sensitivity
  • High gain and high quantum efficiency
  • Instantaneous warm-up and fast response
  • Robust and long lifespan
  • High durability

 

 

Resolve the Dimmest of Signals

 The proprietary optical detection system found inside the NovoCyte Advanteon employs a series of 21 Silicon photomultipliers (SiPM), which have superb detection sensitivity at the photon level.

 The novel design provides highly discrete management of each fluorescent channel, superior optical detection, and cross-talk between channels is completely minimized.

 The NovoCyte Advanteon also has an automatic optical filter recognition feature, which allows users to efficiently change filter configurations, as needed by the user. The system has a fail-safe hardware feature, which conveniently alerts the user to any improperly arranged filter configurations. This feature mitigates against potential hardware errors during system set-up, which makes it ideal for a multi-user environment.

 

 

Resolve Smalest Particles

 NovoCyte Advanteon’s scatter detection optics and signal processing electronics have been optimized to resolve particles of size down to 0.1 μm. With such superior resolution, platelets, bacteria, and various sub-micron particles can be readily identified and analyzed.

 

NovoExpress Software: Streamlined Set-up and Data Analysis

  • One software interface for all: sample acquisition and data analysis
  • Analyze acquired data in real-time during flow experiment
  • Customizable statistical parameters with live updates when running samples
  • Powerful compensation tools and convenient adjustments
  • Batch analysis and reporting
  • Easily create publication-quality figures with customizable plot scales, fonts, and legends
  • Export as FCS (3.0, 3.1) or CSV files, import FCS files for analysis

 

 

 

   

This was a first overview. Now, it's your turn - expand capabilities in your lab, contact us at any time:

 

 

Fluorochrome Spectra Viewer

Spectra Viewer is a tool for interactively viewing and finding fluorochromes and their compensation trade-offs based on flow cytometer laser and filter settings to customize a multicolor flow experiment.

Follow the link to view NovoCyte specific fluorochrome signal and spillover across each channel.: SPECTRA-VIEWER

 

 

 

 

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