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CASY TTC

Kapillaren

Wählen Sie zwischen einer (150; Arbeitsbereich von 3,2 bis 120 µm) oder drei Kapillaren (45/60150; Gesamtarbeitsbereich von 0,7 bis 120 µm).

Manufacturer: OLS OMNI Life Science

Order number: 5651719

Accessories CASY Systems

Brochures & Applications

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CASY TTC

+ + + New Data Acquisition and Analysis Capabilities using Tablet, Laptop or PC + + + 

+ + + More and better measuring routines, enabling data evaluation and organization tools + + +

+ + + Updates available for CASY 2 models. learn more: casy.ols-bio.de + + + 

 

Cell Counter + Analyzer for the high end research lab with extended demand for reproducibility, sample throughput, documentation of results and for research areas that work under GLP/GMP regulations.

Areas of Application

  • Reliable multi-parameter QA on cell cultures at lowest cost
  • Simultaneous determination of cell count, size distribution, cell volume, aggregation and cell debris in less than 10 seconds, avoiding any dyes
  • Need for highest accuracy and reproducibility, which dye-based methods and systems with autosampler (= longer waiting times) cannot achieve
  • Laboratories with several users and different applications 
  • Need for highest quality documentation and evaluation of results
  • Controlled work environment and SOPs that require compliance with GLP/GMP


Three different capillary sizes enable a measuring range reaching from 0.7 µm up to 120 µm:

Capillary Ø [µm]Size Range [µm]Application Areas
45 0.7 - 30

Debris, Bacteria, Spores

60 1.2 - 40

Yeast, Algae, Erythrocytes, Pollen, Plankton, Parasites, Sperms

150 3.2 - 120 Cell lines, Primary mammalian cells, white blood cells, stem cells, fish cells, large algae and protozoa, plant cells and protoplasts

For CASY TTC a large and continuously growing library of reference data is available. The required software CASY®datadoc comes with every system. Please see the following link for some key examples: Brochures & Applications.

CASY: Budget-friendly Benefits to Your Cell Analysis

  • Dye-free, non-invasive determination of the cell activity via ECE® (electric current exclusion method): produces correct data on viability and vitality even for very sensitive cells and partly damaged cells
  • Aggregation factor calculation based on particle volume: Correct cell count even if large cell clusters are present
  • No single use consumables like counting slides or counting capillaries: very low cost per count

Designed for the Demanding Quality Control

CASY TTC was developed especially to meet the requirements in QA of cell culture in research labs as well as in process development and production control. It fulfills the high standards of documentation quality and comprehensive data evaluation needs and was designed to meet the special requirements of labs with a very high number of users and many different applications.

  • A very comfortable windows-based user interface, menus driven and equipped with a full size computer monitor for displaying results and the high res size distribution histograms (1024 size channels)
  • Unlimited storage of cell line/application -specific set-ups
  • LAN interface to connect the systems to data storage and network printers
  • Full flexibility for data evaluation: either directly on the embedded PC or externally using CASY®datadoc software
  • Full GLP/GMP/21 CFR 11 compliance, 3 user levels (guest, user, admin), checksum-secured file formats, electronic signatures and audit trails
  • Simple validation of Analyzer system and embedded PC (IQ/OQ)
  • Dimensions (HxWxD): 65 cm x 41 cm x 39 cm – w/o keyboard and mouse

CASY® - Technical Overview

Measuring TechniqueCASY TTCASY TTC
measuring principle capillary particle counter with pulse area analysis
Parameters determined Cell size distribution, cell count, cell concentration, cell viability, cell aggregation, cell debris, cell volume (peak), average cell diameter
Determination of size dsitribution Digital pulse count/area analysis, 1 Million data points per second, equals several 100 data points per cell
 
Viability measurement

Electrical Current Exclusion (ECE®)
Dye-free, non-invasive determination of the membrane permeability

Aggregation factor determination Based on the volume of the single free cell, resolves clusters from 2 up to several hundred cells
Concentration determination Counting volume controlled opto-electronically, maintenance free, calibrated for life time

 

Performance DataCASY TTCASY TTC
Reproducibility

Typical Std. Deviation = +/- square root (number of counted particles) e.g.: 10 000 cells = +/- 2%

Sensitivity >= 100 Particles/ml
Measuring Range

0,7 - 120µm (with all 3 capillaries)
 

Dynamic Ranges

1 : 70 000 in volume corresponding to 1 : 40 in diameter

Resolution of
size distribution

512 000 channels, linearized by volume

Resolution of
size distribution display

400 channels, linearized by volume 1024 channels, linearized by diameter
Typical measuring times


150µm capillary: 10 sec., 60µm capillary: 25 sec, 45µm capillary: 45sec

Typical sample volume required 5 - 100µl
Storage capacity of cell specific setups 20 unlimited
Cleanroom Qualification No fan, certified for cleanroom class A

 

Quality AssuranceCASY TTCASY TTC
Self-Test

Checks all relevant parameters upon power-on (POST) and before every measurement

Sensor control Continuous electronic monitoring of capillary
System Self-Protect Automatic recognition of operating errors, error message display 
Calibration

Calibration certified ex works, life time calibration,
guarantees identical results from all CASY systems

QC Standards

GLP/GMP compliant, signature required

GLP/GMP compliant and meeting 21 CFR 11 (electronic signature)
Audit Trails no yes – full independent audit trails recorded for: System-, File- and Error events

 

CommunicationCASY TTCASY TTC

Data Entry -
Results display

Separate keypad with graphical display and 13 keys Menu driven software, Win CE®, color screen and embedded PC
Data format ASCII Binary, checksum protection
Data export Structured ASCII text, for import into any other software (e. g. MS Excel)
Interfaces

Data: 2 x RS 232 (DB9),
Printer: Parallel Centronics (DB25)

Raw data: 2 x RS 232 (DB9)
Mouse, Keyboard: USB
Printer: USB, LAN
File storage: CF-Card, LAN

PC-Software CASYexcell (Viewer) CASYdatadoc (Editor)

 

Running CostsCASY TTCASY TTC
Per single measurement approx. 17 Cent/measurement (triple counting)
Service interval 2 years recommended

 

Onward links to "CASY TTC"

Further products by OLS OMNI Life Science
 
Systemkomponenten

CASY TTC

Capillary 150 µm

CasyCups

CasyTon

CasyBlue

CasyClean

Software CasyDatadoc

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