+++ New Application Note: iPS Cell Propagation: Quick & Easy Cell Quality Control +++



Wählen Sie zwischen einer (150; Arbeitsbereich von 3,2 bis 120 µm) oder drei Kapillaren (45/60150; Gesamtarbeitsbereich von 0,7 bis 120 µm).

Manufacturer: OLS OMNI Life Science

Order number: 5651697

Accessories CASY Systems

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Cell Counter + Analyzer for research labs with high sample throughput and/or GLP certification demanding highest degree of reproducibility

Areas of Application

  • Reliable multi-parameter QA on cell cultures at lowest cost
  • Simultaneous determination of cell count, size distribution, cell volume, aggregation and cell debris in less than 10 seconds, no dyes used
  • Need for highest accuracy and reproducibility, which dye-based methods and systems with autosampler = longer waiting times cannot achieve


Three different capillary sizes enable a measuring range reaching from 0.7 µm up to 120 µm:

Capillary Ø [µm]Size range [µm]Application areas
45 0,7 - 30

Debris, Bacteria, Spores

60 1,2 - 40

Yeast, Algae, Erythrocytes, Pollen, Plankton, Parasites, Sperms

150 3,2 - 120

Cell lines, Primary mammalian cells, white blood cells, stem cells, fish cells, large algae and protozoa, plant cells and protoplasts

For the CASY® TT a large and continuously growing library of reference data is available. The required software CASY®excell comes with every system. Please see the following link for some key examples: cellcounting.de/cell-library

CASY: Budget-friendly Benefits to Your Cell Analysis

  • Dye-free, non-invasive determination of the cell activity via ECE® (electric current exclusion method): produces correct data on viability and vitality even for very sensitive cells and partly damaged cells
  • Aggregation factor calculation based on particle volume: Correct cell count even if large cell clusters are present
  • No single use consumables like counting slides or counting capillaries: very low cost per single count experiment

Designed for the Demanding Quality Control

The CASY TT was developed primarily for use in cell culture research in universities and in pharmaceutical and biotech research companies and comes as a stand-alone unit. As such the space requirements are very small and it fits into even the smallest lab. It offers the following key features:

  • Very easy to operate, separate control panel with graphical display and plastic foil keypad
  • Display of size distribution histogram and key results on the same screen mask
  • Stores up to 20 parameter sets for different cell types
  • Dimensions (HxWxD): 39 cm x 31 cm x 39 cm, excl. keypad
  • Results can be stored externally by using the CASYworX software; requires MS Excel

CASY - Technical Overview

Measuring TechniqueCASY TTCASY TTC
measuring principle capillary particle counter with pulse area analysis
Parameters determined Cell size distribution, cell count, cell concentration, cell viability, cell aggregation, cell debris, cell volume (peak), average cell diameter
Determination of size dsitribution Digital pulse count/area analysis, 1 Million data points per second, equals several 100 data points per cell
Viability measurement

Electrical Current Exclusion (ECE®)
Dye-free, non-invasive determination of the membrane permeability

Aggregation factor determination Based on the volume of the single free cell, resolves clusters from 2 up to several hundred cells
Concentration determination Counting volume controlled opto-electronically, maintenance free, calibrated for life time


Performance DataCASY TTCASY TTC

Typical Std. Deviation = +/- square root (number of counted particles) e.g.: 10 000 cells = +/- 2%

Sensitivity >= 100 Particles/ml
Measuring Range

0,7 - 120µm (with all 3 capillaries)

Dynamic Ranges

1 : 70 000 in volume corresponding to 1 : 40 in diameter

Resolution of 
size distribution

512 000 channels, linearized by volume

Resolution of 
size distribution display

400 channels, linearized by volume 1024 channels, linearized by diameter
Typical measuring times

150µm capillary: 10 sec., 60µm capillary: 25 sec, 45µm capillary: 45sec

Typical sample volume required 5 - 100µl
Storage capacity of cell specific setups 20 unlimited
Cleanroom Qualification No fan, certified for cleanroom class A


Quality AssuranceCASY TTCASY TTC

Checks all relevant parameters upon power-on (POST) and before every measurement

Sensor control Continuous electronic monitoring of capillary
System Self-Protect Automatic recognition of operating errors, error message display 

Calibration certified ex works, life time calibration, 
guarantees identical results from all CASY systems

QC Standards

GLP/GMP compliant, signature required

GLP/GMP compliant and meeting 21 CFR 11 (electronic signature)
Audit Trails no yes – full independent audit trails recorded for: System-, File- and Error events


CommunicationCASY TTCASY TTC

Data Entry - 
Results display

Separate keypad with graphical display and 13 keys Menu driven software, Win CE®, color screen and embedded PC
Data format ASCII Binary, checksum protection
Data export Structured ASCII text, for import into any other software (e. g. MS Excel)

Data: 2 x RS 232 (DB9), 
Printer: Parallel Centronics (DB25)

Raw data: 2 x RS 232 (DB9)
Mouse, Keyboard: USB
Printer: USB, LAN
File storage: CF-Card, LAN


CASYexcell (Viewer)

CASYworX (View & Edit)

CASYdatadoc (Editor)


Per single measurement approx. 17 Cent/measurement (triple counting)
Service interval 2 years recommended


Onward links to "CASY TT"

Further products by OLS OMNI Life Science


Capillary 150 µm





Software CasyExcell

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