Multi-Parameter - Accurate - Reproducible
Globally, thousands of users, industrial and academic, trust CASY, the legend in cell counting. Whether working with mammalian cells, yeast, bacteria or one of many other cell types, CASY users rely on the unmatched precision, reproducibility and accuracy of the instrument combined with easy and intuitive use.
The CASY technology uses the current exclusion method: particles and cells passing through a defined size pore generate a resistance signal varying with their size and conductivity. Live cells with a polarized membrane generate a high resistance signal, whereas dying or dead cells display a much lower resistance due to their depolarized or fully disrupted cell membrane. The signal of one cell passing the pore is resolved with a high frequency of 1 MHz, and hence the area under the curve = the Integral can easily be calculated, the integral being the only parameter directly proportional to the cell size. As the integral is seriously different with live cells, dead cells and debris, the particle sizes and their distribution can be recorded and analyzed very precisely. One additional benefit is the large dynamic measuring range (e.g. 150µ capillary: 3.2 – 120 µm = from debris via dead/live cells to cell aggregates of several 100 cells size), which cannot be reached by any other method of cell size analysis. By limiting the number of count events, CASY works without any mathematical approximations to deal with multiple particles passing the capillary simultaneously, every cell or particle is analyzed as a single count event.
We have been using CASY for the fast and reliable determination of cell concentrations and cell diameters of our cell cultures for 20 years now. We are still impressed by the high reproducibility of the measurements and the simple, intuitive operation of the device. The associated software offers great options for managing and displaying the measurement results graphically.
We study the regulation of cancer cell growth by signaling and metabolism. CASY is the ideal system for us to precisely measure proliferation and cell size of a wide range of cell lines. In this context, the new software makes instrument operation as well as data visualization and organization easy and convenient.
We use CASY cell counter for toxicological studies at a single animal level. The CASY cell counter proved to be an accurate and sensitive instrument for ecotoxicological applications allowing fast, simple and label-free measurements.
Cytotoxicity Assays using CASY enable us to perform fast, simple and reliable assessment of anti-cancer drug regimens and the subsequent chemosensitivity of carcinoma cell lines. A major advantage of CASY is that debris and dead cells can be masked in the analysis by a simple cell line specific setup and only viable cells and their aggregates are considered. However, as a complete cell status is recorded, also dead cells and debris data are available at any time.
|The sensor is directly immersed into the sample solution||Grants no errors, no losses of cell Material by sedimentation , correct analysis of cell aggregates|
|Dye-free, non-invasive measurement of cell number and viability||Accurate results for viability of sensitive and even partially damaged cell suspensions, free nuclei are determined as dead cells|
|Large dynamic measuring range||Every single analysis covers the full range from Debris, to dead and live cells and includes large aggregates of living cells|
|High resolution size distribution dis-played as histogram with over 400 size channels||All results at one single glance|
|Aggregation factor calculated from particle volume||Correct results also from highly aggregative cell types|
|No single-use counting chambers or dedicated slides necessary||Lowest possible cost/analysis ratio|
|Rapid Measurement – from only 10 sec to 1 min per measurement||Immediate results, no risk of measurement artefacts from sedimentation while waiting in an autosampler|
Webinar | Webinar | 2 pm
The webinar will provide insight in the principle and the advantages of electronical measurements using CASY. Some of the advantages will be demonstrated on particular analysis of samples using the new CASY software. Examples will include • PMBC – differential detection of Lympho- and Monocytes • iPSC – how to measure accurately if single cells are difficult to generate and aggregation gets a problem • other The webinar will take about 60min of presentation and an additional 30 min for question and answers.Read more ›
Fair | Messe Munich | 9 am
POSTPONED to 19. - 22.10.2020!!! Smart Solutions from Cell Culture and Counting to Real Time Cell Analysis and Live Cell Imaging are offered by OLS OMNI Life Science at Analytica 2020. Visit our booth 210 in hall A3 to get the latest news for your powerful cell analysis. Join us for a drink and meet the OLS team.Read more ›
|CASYton||5651808||Isotonic measurement buffer for all CASY models. Politainer for easy filling of consumption quantities, 1x 10 L||Quote||Order now|
|CASYcups||5651794||Measuring vessels for all CASY models. For dilution of the samples to be measured with CASYton and performing the cell count. 1 pack = 1800 cups||Quote||Order now|
|CASYclean||5651787||Capillary and system cleaning agent for all CASY models. To be used for acute decontamination and routine cleaning. 1 x 500 mL||Quote||Order now|
|CASYblue||5651760||Solution for controlled killing of cell culture cells for accurate adjustment of the left cursor evaluation, 4 x 30 mL||Quote||Order now|
Simple but reliable: How even novice users receive best results with CASY
Ecotoxicology: Algae Feeding Rates of Daphnia magna
The Cytotoxicity of Anti-Cancer Drug Combinations
Instructions for setting system out of operation, no use for > 2 week
Overview of all available CASY results
Set of CASY results for algea and protozoa
Set of CASY results for bacteria
Set of CASY results for blood cells
Set of CASY results for various cell lines
Set of CASY results for parasites
Set of CASY results for platelets
Set of CASY results for primary cells
Set of CASY results for stem cells