Multi-parameter - Accurate - Reproducible
Globally, thousands of users, industrial and academic, trust CASY, the legend in cell counting. Whether working with mammalian cells, yeast, bacteria or one of many other cell types, CASY users rely on the unmatched precision, reproducibility and accuracy of the instrument combined with easy and intuitive use.
The CASY technology uses the current exclusion method: particles and cells passing through a defined size pore generate a resistance signal varying with their size and conductivity. Live cells with a polarized membrane generate a high resistance signal, whereas dying or dead cells display a much lower resistance due to their depolarized or fully disrupted cell membrane. The signal of one cell passing the pore is resolved with a high frequency of 1 MHz, and hence the area under the curve = the Integral can easily be calculated, the integral being the only parameter directly proportional to the cell size. As the integral is seriously different with live cells, dead cells and debris, the particle sizes and their distribution can be recorded and analyzed very precisely. One additional benefit is the large dynamic measuring range (e.g. 150µ capillary: 3.2 – 120 µm = from debris via dead/live cells to cell aggregates of several 100 cells size), which cannot be reached by any other method of cell size analysis. By limiting the number of count events, CASY works without any mathematical approximations to deal with multiple particles passing the capillary simultaneously, every cell or particle is analyzed as a single count event.
We have been using CASY for the fast and reliable determination of cell concentrations and cell diameters of our cell cultures for 20 years now. We are still impressed by the high reproducibility of the measurements and the simple, intuitive operation of the device. The associated software offers great options for managing and displaying the measurement results graphically.
|The sensor is directly immersed into the sample solution||Grants no errors, no losses of cell Material by sedimentation , correct analysis of cell aggregates|
|Dye-free, non-invasive measurement of cell number and viability||Accurate results for viability of sensitive and even partially damaged cell suspensions, free nuclei are determined as dead cells|
|Large dynamic measuring range||Every single analysis covers the full range from Debris, to dead and live cells and includes large aggregates of living cells|
|High resolution size distribution dis-played as histogram with over 400 size channels||All results at one single glance|
|Aggregation factor calculated from particle volume||Correct results also from highly aggregative cell types|
|No single-use counting chambers or dedicated slides necessary||Lowest possible cost/analysis ratio|
|Rapid Measurement – from only 10 sec to 1 min per measurement||Immediate results, no risk of measurement artefacts from sedimentation while waiting in an autosampler|
|CASYton||5651808||Isotonic measurement buffer for all CASY models. Politainer for easy filling of consumption quantities, 1x 10 L,||Quote|
|CASYcups||5651794||Measuring vessels for all CASY models. For dilution of the samples to be measured with CASYton and performing the cell count. 1 pack = 1800 cups,||Quote|
|CASYclean||5651787||Capillary and system cleaning agent for all CASY models. To be used for acute decontamination and routine cleaning. 1 x 500 mL||Quote|
|CASYblue||5651760||Solution for controlled killing of cell culture cells for accurate adjustment of the left cursor evaluation, 4 x 30 mL||Quote|