Workshop
Basics in Flow Cytometry

21^st November 2023 - 22^nd November 2023

Bremen

Come to our Life Science School laboratory here in Bremen and learn the practical and theoretical basics of flow cytometry. Whether you are new to flow cytometry or are looking to refresh your skills, this training program is the perfect opportunity to enhance your knowledge and improve your research capabilities

Topics
Goals
Date, Pricing & Location
Register Here

We are excited to invite you to our upcoming “Basics in Flow Cytometry”-Training at OLS OMNI Life Science. The program will cover a range of topics, including instrument setup, sample preparation, data acquisition and analysis, as well as troubleshooting. Whether you are new to flow cytometry or are looking to refresh your skills, this training program is the perfect opportunity to enhance your knowledge and improve your research capabilities.

Topics of the Course

Basics

Technical basics of Flow Cytometry: Structure and operation of the device
Optical components and detectors: Laser, filters, and photomultiplier
Fluorescence and scatter measurements: What is measured?
Spillover – Spectral overlap
Compensation: What is compensation and why is it important?
Data analysis: Visualization and gating
Creating compensation controls and adjusting compensation matrices
Experiment design: What to consider when planning an experiment?
Controls: How are positive and negative controls used?
What controls are there? Isotype controls, FMO controls.

Applications

Phenotyping: Identification of cell types based on surface markers
Microvesicles: What are they and how are they analyzed?
Apoptosis: How is cell death analyzed using Flow Cytometry?

Practical part

Technical features of the NovoCyte Quanteon
Instrument QC and Troubleshooting
PBMC Staining
Bacteria Staining
Apoptosis

Goals

This course targets people working in a laboratory who are currently not working with flow cytometry. Participants of this course will be introduced or re-introduced to this topic and their knowledge of flow cytometry will be built up to a solid basis by the end. Requirement is the familiarity with conventional workflows and principles of cell culture.

Date, Pricing & Location

Location: Bremen
Course language: English
Date: September 26 10:30 – September 27 15:30 2023

Price: 2.400 Euro ex. MwSt. (2.856 incl. MwSt.)

The course fee includes catering during the course (coffee, drinks, lunch), as well as any schooling material such as prints, as well as the participation certificate after completion.