Classical cell culture had a major limitation where cells are only able to grow in 2D, the XY plane, rather than there natural state in 3D. This is where 3D cell culture comes in. In living organisms, cells exist in a 3D form in 3D microenvironments with highly organized cell-cell and cell-matrix interactions and complex transport dynamics for nutrients and cells. This means that standard classical 2D cell culture can be unreliable for modeling in vivo drug efficacy and toxicity, which are crucial for drug discovery processes.
3D spheroids are better representatives of in vivo tissue in terms of cellular communication and the development of extracellular matrices (ECMs). ECMS allow cells to move within their spheroid similar to the way cells would move in living tissue. This means that spheroids are better models for cell migration, differentiation, survival, and growth.
Organoids on the other hand are a miniaturized and simplified version of an organ produced in vitro in three dimensions that shows realistic micro-anatomy. They are derived from one or a few cells from a tissue, embryonic stem cells or induced pluripotent stem cells, which can self-organize in three-dimensional culture owing to their self-renewal and differentiation capacities. The last 10 years have shown great advancements in organoid research with cerebral organoids, gut, liver, thymus lung, kidney and even retinal organoids available now.
3D cell culture is performed in a bioreactor. Being an ideal partner in cell research, we provide the CERO 3D Incubator & Bioreactor. This revolutionary instrument creating optimal cell culture environment. It offers a special 3D cell culture technology that monitors and controls temperature, pH and carbon dioxide levels. Indeed, this is ideal for stem cells, spheroids, organoids and even tissues.
The CERO 3D Incubator and Bioreactor allows for significant improvement of viability and maturation with minimized apoptosis and necrosis, no shear forces, no requirement for embedding substrate. This indeed means that there is a high level of homogeneity and allowing long-term cultivation for more than one year. Being easy to set-up with simple workflows and minimum hands-on time that can be down to less than 2 minutes per day, the CERO 3D Incubator and Bioreactor significantly reduces your running costs.
In addition, OLS also provides the CEROplate, with ultra-low attachment that simplifies the process for growing 3D aggregates. It features clear wells with a U-bottom to make sample monitoring simple. The unique well geometry of the microplate aids in the formation of an unattached, uniform, round-shaped, single spheroid or organoid in the center of each well. This allows for long-term 3D cell culture over months. CEROplates are compatible with existing imagers, liquid handling and automated stations allowing for perfect data consistency high-throughput applications.