Stem cells – Spheroids – Organoids – Tissues
The CERO 3D Incubator & Bioreactor offers a unique 3D cell culture technology to boost your stem cell, spheroid, organoid and tissue research.
Highest levels of homogeneity and viability in long term cultures are just two benefits CERO 3D will provide.
The CERO 3D Incubator & Bioreactor is a new, revolutionary instrument creating optimal cell culture enviroment by monitoring and controlling temperature, pH and CO2 levels. 1- 4 individually controlled CEROtubes with a volume of up to 50ml provide highest biomass yields in a standardized and reproducible way, with minimum handling requirements. The CEROtubes, with small fins and a flat bottom, enable gentle cultivation conditions with reduced shear stress to your cells.
The CERO 3D Incubator and Bioreactor provides the solution for scale-up and automation platforms, simplification and cost reduction of stem cell expansion projects in biobanks, cell-based drug discovery, toxicity testing and regenerative medicine.
Pluripotent stem cells are directly inoculated as single cells into the CEROtube. During the self-aggregation the cells form homogeneous aggregates which can be expanded over many passages. The biomass increases significantly while only ~2min per day hands on time is required.
The resulting stem cell 3D aggregates can be processed directly for differentiation e.g. organoids or any other downstream application in 3D or 2D assay development.
Pluripotent stem cells expanded in CERO 3D (former name “BioLevitator”) will maintain pluripotency and can be differentiated into all 3 germ layers, as described by Elanzev et. al. 2015; Biotechnol. J. 2015, 10, 1589–1599:
The advent of long-term three dimensional cell culture holds a great promise in disease modeling and drug discovery. The cells kept in a 3D enviroment have the ability to mimic tissue-like structures more efficiently than in traditional 2D monolayer cultures. However, many scientist are struggling with many technical limitations when working with spheroids in long-term cultures.
The CERO 3D Incubator & Bioreactor is a revolutionary technology enabling scientists to perform experiments they were not able to do before.
Spheroids from HepG2 cells (hepatocyte cell line) cultivated in CERO 3D for >80 days.
CERO 3D Incubator & Bioreactor enables growth and maturation of spheroids without necrosis and apoptosis while other technologies fail. Therefore, CERO 3D allows to maintain state-of-the-art 3D cultures of Huh7 hepatic cell line (see figure below). The disposable CEROtubes with Hepa filter allow safe virus experiments.
Cultivating hepatocyte spheroids in CERO considerably improves expansion, differentiation, maturation and hepatic virus infection compared to monolayer culture. Our research takes advantage from healthy cells even from long-term cultures in CERO. Moreover, we are now able to perform 3D long-term culture of human tissue specimen in CERO - a paradigm shift.
Stem cell derived cardiomyocytes gain more and more attention in the field of cardiovascular research, disease modeling and drug development.
The CERO 3D Incubator & Bioreactor platform allows the workflow with stem cell expansion in homogeneous aggregates followed by direct induction into a high number of beating cardiac bodies. The expansion of pluripotent stem cells and subsequent cardiac induction/differentiation in CERO 3D results in a much higher cell quality, homogeneity, integrity and yield compared to traditional orbital shakers.
Purified and homogeneous cardiac bodies generated in CERO 3D Incubator & Bioreactor are a perfect tool for disease modeling and drug discovery. The pure cardiomyocytes can also be used in 2D downstream monolayer applications.
Moreover this 3D cultivation system substantially improves viability, maturation and contractility, therefore provides a reliable tool for cardiovascular therapies.
The CERO 3D Incubator and Bioreactor is a revolutionary technology for production of organoids from pluripotent or adult stem cells. It offers an efficient, standardizable way to generate and maintain high yields of homogeneous organoids used as a tool in cancer research. Remarkably, the organoids have also a predictive capacity for in vivo response.
Increase cellular, structural and functional complexity of organoids.
The generation of gastric organoids is a crucial step in the study of Helicobacter pylori infection and gastric carcinogenesis. The CERO 3D Incubator and Bioreactor allows the generation in a much more efficient, reproducible, physiological and cost efficient way compared to any other approach
The progress in the development of molecular target therapies has improved the outcome in “in vivo”, however it is because of the tumor heterogeneity in patients that limits the efficiency of those drugs. Thus, to increase the relevance of “in vitro” models, it is particularly important to develop a reliable platform for growing primary tumor cells in a 3D in vitro model, like patient-derived xenografts or organoids. The CERO 3D Incubator and Bioreactor evolving a state-of-the-art dynamic culture system, accelerates precision medicine of primary tumor cells. The CERO 3D offers an efficient and reproducible approach to grow and maintain viable cancer cells in a CERO 3D Incubator and Bioreactor.
Our group is focusing on primary tumor cells grown on 3D polymer scaffolds. For that we are using CERO 3D to develop a model of drug development and precision medicine. It is particularly important that the cells are viable and proliferating in 3D complex structures as seen in tumor tissues. With the CERO 3D we are implementing and developing tools for patient-specific tumor therapy. Furthermore, the cancer cells isolated from fresh tumor biopsies make treatment optimization possible before a therapy is initiated. Here, CERO 3D incubator & bioreactor enables us to develop personalized medicine in a 3D cell culture model.
The CERO 3D Incubator and Bioreactor allows to combine expansion of pluripotent stem cells with the induction of differentiation and cultivation of organoids. Normally, this is a time and effort consuming, multistep process and almost impossible to standardize. The CERO 3D Incubator & Bioreactor helps you to overcome these challenges.
Pluripotent stem cell expansion combined with generation of high yield Neuronal stem Cells (NSC) aggregates.
The CERO 3D Incubator and Bioreactor is easy to operate and allows generation of homogeneous aggregates NSC in high biomass (~1g per tube) in short time.
Exhibition | EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg | 10 am
Microglia are a resident macrophage of the central nervous system at the intersection of immunology and neuroscience. They have gained increasingly more attention over the past decade as more research shows that they are key contributors to neural circuit development and function. There is also increasing evidence that microglia are also important in the etiology of many nervous system diseases. This workshop will highlight the many important functions of microglia to nervous system physiology and function, the newest and most exciting literature and will bring together researchers using state-of the art imaging, genomics and molecular biology to study these enigmatic cells.
The brand new CERO 3D application handbook
Generation of HepaRG Spheroids in the CERO
Scientific Poster: "Establishment and validation of the 3D cultivation system“CERO” for hepatic spheroids to study viral hepatitis", presented at the 2018 EMBL Symposium "Organoids: Modelling Organ Development and Disease in 3D Culture"
Expansion Protocol Pluripotent Stem Cells (iPSC and ESC)
Differentiation Protocol Organoids from Pluripotent Stem Cells
Instructions for setting system out of Operation, no use for > 4 weeks