Immuno-Oncology also known as immunotherapy is a form of cancer treatment that uses the power of the body’s own immune system to prevent, control, and eliminate cancer. Immune-Oncology involves educating the immune system to recognize and attack specific cancer cells, as well as boost immune cells to help them eliminate cancer.
Immuno-Oncology has been widely recognized and is one of the fastest growing fields in biological sciences. American immunologist James Allison and Japanese immunologist and Tasuku Honjo shared the Nobel Prize in Medicine in 2018 for their discovery of cancer therapy by inhibition of negative immune regulation.
If you are in the field of immunotherapy or starting out, OLS is your partner every step of the way.
Both immune cells and tumor cells have virtually limitless behaviors and profiles. Along with viruses that can be used as oncolytics or gene delivery vectors for immunotherapy xCELLigence® RTCA technology allows detection of minimal cellular changes, involving strength of adhesion, cell number, cell morphology and cell-cell-contacts. xCELLigence RTCA can also determine viral infections of cells and detect virus titers as well as the determination of neutralizing antibodies.
Whether it is cellular behavior, viral infection or virus-based therapeutics, xCELLigence RTCA provides deep insight into whole kinetics of cellular behaviour with high sensitivity, without any cell-labelling and with only minimal hands-on-time.
Flow cytometry has rapidly evolved over the past few decades. For every step of the way whether bioengineering of T lymphocytes for immunotherapy, and immune checkpoint blockades, monitoring to drug distribution to tumor cells, as well as immune cell stimulation regulation. Agilent’s NovoCyte Flow Cytometers known for their fast and sensitive detection of the target labeled with up to 30 fluorescent colors. With the high-throughput capability, identify the lead through your targets and validate their efficacy. Following these steps, perform the perfect analysis of your target whether it is an antibody, a protein, or a small molecule. In collaboration with BioLegend, Agilent also developed a Cytokine detection utilizing a 27-plex cytokine detection kit. It is actually a 13-plex and 14-plex kits used together, but the idea is to get an overall assessment of immune status by measuring these various cytokines.
If you are getting into the business of single cell analysis, our Namocell Single Cell Dispensers make single cell isolation and sorting effortless. These benchtop devices use proprietary.
Microfluidics technology to sort and dispense single cells directly into 96-well or 384-well plate. They simplify the wide variety of single cell applications to study immune cells and tumor cells every step of the way. This includes cell line development, the Namocell Single Cell Dispenser is able to dispense single cells into 90% of the wells. Using fluorescence sorting capabilities, Namocell Single Cell Dispenser is able to isolate single cells that have high antibody production. Single cell genomics with its great advancements in the past decade revolutionized the study of cellular heterogeneity. Both immune cells and tumor cells have a wide range of individual distinct molecular profiles to be investigated. Advancements in tools such as RNA sequencing of single cells (scRNA-seq) has been used to identify and define the variety of cell subtypes. The Namocell Single Cell Dispenser provides a simple, affordable, and flexible platform for any single cell study. The ability to sort and isolate only live cells or a sub-population of cells provide new opportunities for single cell studies.
Therefore, our NovoCyte Flow Cytometers and Namocell Single Cell Dispensers can greatly complement your efforts for monoclonal antibody development, T-cell engineering, CRISPR selection, synthetic biology, circulating tumor cells (CTCs) isolation, and single cell profiling. Our instruments are affordable, user-friendly, and requires minimal to no training to operate. They are ideal for every step of the way for pre-clinical and clinical cancer immunotherapy studies.
High-demand fields like immuno-oncology, necessitate innovative technologies. For this purpose, OLS constructs the intricate CASY technology – CASY uses a sensitive electrode paired with a highly calibrated capillary to capture the distribution of cell volumes in dependance of the individual electrical resistance of each single cell in the sample. The cell volume provides an easy understanding of cell number, viability, cells affected by infection, differentiating stem cells, or cell type. There is no other analysis method providing this much information in such a short amount of time.
Immune cells and tumor cells can be therefore easily examined. Factors like proliferation behavior, developmental stage and even viral infections become quantifiable simultaneously – all the while abstaining from laborious factors such as sample staining and lengthy preparation times.