Cell Analysis ELISA Kits

ELISA-Kits by AuthentiKine™

Proteintech ELISA kits – simple and elegant solution for your immunoassay needs

Ultra-sensitive kits between 10X and 1000X more sensitive than kits sold by leading competitors
The only kits made using actual human proteins from state-of-the-art HumanKine® human Cell-expressed cytokines and growth factors.

Proteintech’s AuthentiKine™ Sandwich ELISAs offer unmatched sensitivity compared to conventional ELISA kits in the market. AuthentiKine™ ELISA kit development starts with production of HumanKine® immunogens, which are human proteins with native glycosylation and processing whereas most other ELISA kit manufacturers use immunogens synthesized in a prokaryotic system.

Due to the highest quality immunogens in the industry, the capture and detection antibodies have the highest affinity for human proteins. To best mimic the human sample analyte for optimizing the ELISA parameters, HumanKine® proteins are used as the standard. AuthentiKine™ ELISA kits are well validated in natural samples like human serum and plasma.

Cell Analysis ELISA Kits

The procedure is straightforward and allows you to detect and quantify your target protein, reliably and accurately, in a range of biological samples in the space of a morning or afternoon. Proteintech’s ELISA kits are based on the two-site sandwich immunoassay principle, in which two highly specific, non-competitive antibodies are used in the detection of a target analyte: one for capture, and one for detection.

Briefly, steps involved in making Proteintech’s sandwich ELISA kits include:

Careful selection of antibody pairs to ensure optimum signal
Calibration with NIBSC/WHO Standards
Testing with multiple sample types, e.g., sera, plasma, cell culture supernatant
Testing of intra- and inter-assay precision
Linearity testing
Recovery testing
Kit stability testing

ELISA sensitivity: AuthentiKine™ vs Competitors

Sensitivity pg/mL
Product Name	AuthentiKine™	Leading competitor
Human IFN-gamma ELISA kit	0.4 pg/mL	8 pg/mL
Human Cystatin C ELISA kit	10.5 pg/mL	102 pg/mL

Human APOL1 ELISA Kit

APOL1 is involved in the formation and efflux of cholesteryl esters and lipid transport. Proteintech offers a human-specific APOL1 ELISA kit, with high sensitivity and broad assay range.
The KE00047 kit is a quantitative measurement of human APOL1 protein in serum and plasma. It has recently been cited in top scientific publications as a useful tool regarding HIV, CKD, and frontotemporal dementia studies. ¹ ² ³
• Tests: 1 x 96 well plate, Sandwich ELISA for Serum, plasma
• Assay Time: 3.5 hours
• Sensitivity: 70 pg/mL
• Range: 156 – 10000 pg/mL
• Regulatory: Research use only

Standard Curve

References

¹ Vascular smooth muscle cells contribute to APOL1-induced podocyte injury in HIV milieu, (PMID: 25796344).

² Novel diagnostic cerebrospinal fluid biomarkers for pathologic subtypes of frontotemporal dementia identified by proteomics, (PMID: 27239539).

³ A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease, (PMID: 28650456).

Products

Human TDP-43 ELISA Kit

520,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | EntrezGeneID: 23435 | Range: 6.25-400 ng/mL

Human TNFR1 ELISA Kit

269,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | EntrezGeneID: 7132 | Range: 3.9-250 pg/mL

Human CXCL16 ELISA Kit

269,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | EntrezGeneID: 58191 | Range: 7.8-500 pg/mL

Human ST2 ELISA Kit

269,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | AEntrezGeneID: 9173 | Range: 31.25-2000 pg/mL

CD Molecular
ELISA Kit	Page	ELISA Kit	Page	ELISA Kit	Page
CD31	6	CEACAMS	6	PD-11	7
CD3E	6	PD1	7

Cytokines
ELISA Kit	Page	Elisa Kit	Page	Elisa Kit	Page
Adiponectin	7	GKN1	10	MMP-13	13
BIRC5	8	GMFB	10	NCALD	13
CCL4	8	IFNA1	11	SERPINA3	13
CHGA	8	IFN-gamma	11	TGF-beta1	14
F12	9	IL 1-beta	11	TGF-beta1	14
FGF12	9	IL2	12	TLE3	14
FN1	9	MCP-1	12	TNF-alpha	15
GM-CSF	10	MMP-2	12