Cell Analysis ELISA Kits
Made with HumanKine human cell-expressed proteins

ELISA-Kits by AuthentiKine™

Proteintech ELISA kits – simple and elegant solution for your immunoassay needs

  • Ultra-sensitive kits between 10X and 1000X more sensitive than kits sold by leading competitors
  • The only kits made using actual human proteins from state-of-the-art HumanKine® human
  • Cell-expressed cytokines and growth factors.

Proteintech’s AuthentiKine™ Sandwich ELISAs offer unmatched sensitivity compared to conventional ELISA kits in the market. AuthentiKine™ ELISA kit development starts with production of HumanKine® immunogens, which are human proteins with native glycosylation and processing whereas most other ELISA kit manufacturers use immunogens synthesized in a prokaryotic system.

Cell Analysis ELISA Kits

Due to the highest quality immunogens in the industry, the capture and detection antibodies have the highest affinity for human proteins. To best mimic the human sample analyte for optimizing the ELISA parameters, HumanKine® proteins are used as the standard. AuthentiKine™ ELISA kits are well validated in natural samples like human serum and plasma.

The procedure is straightforward and allows you to detect and quantify your target protein, reliably and accurately, in a range of biological samples in the space of a morning or afternoon. Proteintech’s ELISA kits are based on the two-site sandwich immunoassay principle, in which two highly specific, non-competitive antibodies are used in the detection of a target analyte: one for capture, and one for detection.

Briefly, steps involved in making Proteintech’s sandwich ELISA kits include:

  • Careful selection of antibody pairs to ensure optimum signal
  • Calibration with NIBSC/WHO Standards
  • Testing with multiple sample types, e.g., sera, plasma, cell culture supernatant
  • Testing of intra- and inter-assay precision
  • Linearity testing
  • Recovery testing
  • Kit stability testing

ELISA sensitivity: AuthentiKine™ vs Competitors

Sensitivity pg/mL
Product Name AuthentiKine™ Leading competitor
Human IFN-gamma ELISA kit 0.4 pg/mL 8 pg/mL
Human Cystatin C ELISA kit 10.5 pg/mL 102 pg/mL

Human APOL1 ELISA Kit

APOL1 is involved in the formation and efflux of cholesteryl esters and lipid transport. Proteintech offers a human-specific APOL1 ELISA kit, with high sensitivity and broad assay range.
The KE00047 kit is a quantitative measurement of human APOL1 protein in serum and plasma. It has recently been cited in top scientific publications as a useful tool regarding HIV, CKD, and frontotemporal dementia studies. ¹ ² ³
• Tests: 1 x 96 well plate, Sandwich ELISA for Serum, plasma
• Assay Time: 3.5 hours
• Sensitivity: 70 pg/mL
• Range: 156 – 10000 pg/mL
• Regulatory: Research use only

 

Standard Curve

APOL1 Concentration

References

¹ Vascular smooth muscle cells contribute to APOL1-induced podocyte injury in HIV milieu, (PMID: 25796344).

² Novel diagnostic cerebrospinal fluid biomarkers for pathologic subtypes of frontotemporal dementia identified by proteomics, (PMID: 27239539).

³ A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease, (PMID: 28650456).

Products

ELISA Kit TDP-43

Human TDP-43 ELISA Kit

520,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | EntrezGeneID: 23435 | Range: 6.25-400 ng/mL

Human TNFR1 ELISA Kit

Human TNFR1 ELISA Kit

520,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | EntrezGeneID: 7132 | Range: 3.9-250 pg/mL

Human CXCL16 ELISA Kit

Human CXCL16 ELISA Kit

350,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | EntrezGeneID: 58191 | Range: 7.8-500 pg/mL

Human ST2 ELISA Kit

Human ST2 ELISA Kit

350,00 €
Storage Temperature: 2-8℃ for 6 months or -20℃ for 12 months | Applications: Sandwich ELISA | Reactivity: Human | AEntrezGeneID: 9173 | Range: 31.25-2000 pg/mL

 

CD Molecular
ELISA Kit Page ELISA Kit Page ELISA Kit Page
CD31 6 CEACAMS 6 PD-11 7
CD3E 6 PD1 7
Cytokines
ELISA Kit Page Elisa Kit Page Elisa Kit Page
Adiponectin 7 GKN1 10 MMP-13 13
BIRC5 8 GMFB 10 NCALD 13
CCL4 8 IFNA1 11 SERPINA3 13
CHGA 8 IFN-gamma 11 TGF-beta1 14
F12 9 IL 1-beta 11 TGF-beta1 14
FGF12 9 IL2 12 TLE3 14
FN1 9 MCP-1 12 TNF-alpha 15
GM-CSF 10 MMP-2 12
Interleukin
ELISA Kit Page ELISA Kit Page ELSA Kit Page
IL2 15 IL8 19 IL13 20
IL4 18 IL10 19 IL17A 20
IL5 18 IL12 p40 19 IL28A 21
IL6 18 IL12 p70 20 PRE-IL18 21
Membrane Protein
ELISA Kit Page ELISA Kit Page ELISA Kit Page
ABCD1 21 CD71 23 MHC Class 1 24
ADAM12 22 CNPY2 23 NINJ2 24
APOL1 22 CYCS 23 TNF-alpha 25
CD13 22 HSPD1
Other
ELISA Kit Page ELISA Kit Page ELISA Kit Page
Albumin 25 HSP70 27 Mamma- 28
APOD 25 HSP90 27 globinA
CYB5R3 26 IDH1 27 P21 28
FKBPL 26 JNK 28 28 SMN2 29
HDAC1 26

See also

For more related information, take a look at the products under the following links:

Antibodies for Flow Cytometry

Setting the benchmark for Flow Cytometry Powerful – Broad range - Multiple Fluorophores

Proteintech Flow Antibodies

Cytokines and Growth Factors

Highest grade at lowest price – Authentic human proteins

Proteintech Growth Factors

Contact

Fabian Stautz
Inside Sales

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