BioBanking Solutions

OLS focusses on solutions for Biobanking workflows starting from your sample:

• Blood / PBMC
• Cell (Cell lines, Stem- or Hybridoma Cells)
• Tissue

PBMC Biobank

• Separation:

Peripheral Blood Mononuclear Cells (PBMCs) are isolated from Blood using HiSep^TM Lymphocyte Separation Media (LSM1077). Other Separation Media are available as well. For details see HiSep^TM Lymphocyte Separation Media.

• Preparation:

For storage of PBMC/Lymphocyte special cryo-media are available. CryoXL^TM Cell Freezing Medium – DMSO with Serum (TCL093-50ML) or Serum Free (TCL098-50ML) are recommended. For More Details see CryoXL^TM – Cryopreservation.

• Storage:

Cryo Vials are available in volumes ranging from 0.5 to 5ml designed for storage in liquid nitrogen and ultra-low freezers. For details see Cryo Vials.

• Counting & Analysis:

CASY CAN DO

CASY Cell Counter & Analyzer is the perfect counter to analyze quality of samples before freezing and after thawing. In case of PBMC, not only cell number and viability are measured but the volume-based size distribution allows to understand variation in share between lymphocyte and monocyte. See CASY Cell Counter & Analyzer for details.

More analytical solutions are available using Flow Cytometry and Imaging

Cells Biobank

Requirements for biobanking of cells vary based on type of cells. Especially concerning expansion of cells, beside the classical solution of static 2-dimensional expansion, superior solutions are available for 3D cell culture, the CERO 3D Incubator and Bioreactor.

• Expansion:

A comprehensive portfolio of products for cultivation of cells ranging from classical to specialty media, from supplements and chemicals to cell culture systems.
For details download our new HiMedia catalog and contact us.

The novel CERO 3D Incubator and Bioreactor is the perfect solution for expansion of pluripotent stem cells as well as other cell types. Expansion in 3D improves yield, reduces cost and at the same time support maintaining pluripotency of stem cells. For details see CERO 3D Incubator and Bioreactor.

• Dissociation Reagents:

A broad range of dissociation reagents – enzymatic or non-enzymatic – allow to choose the best solution for each application. Same time animal component free reagents are available. For details visit Dissociation Reagents.

• Preparation:

Different cell types have different requirements of cryo media. The CryoXL^TM Cell Freezing Medium product line provides special solutions for sensitive-, stem- or hybridoma cells containing DMSO or Glycerin. Serum Free Media are available too. For more details see CryoXL^TM – Cryopreservation.

• Storage:

Cryo Vials are available in volumes ranging from 0.5 to 5ml which are designed for storage in liquid nitrogen and ultra-low freezers. For details see Cryo Vials.

• Counting & Analysis:

CASY Cell Counter & Analyzer

CASY Cell Counter & Analyzer combines precision and reproducibility of results, with highest flexibility for type of sample and quality control. Even critical cells, like induced pluripotent stem cells (iPSC) or embryonic stem cells (ESC) can be analyzed using CASY’s unique volume-based aggregation correction. See CASY Cell Counter & Analyzer for details.

More analytical solutions are available using Flow Cytometry and Imaging

Tissue Biobank

Tissue- and Cell Dissociation Regents, 3D Organoid formation and cultivation, Cryo-Media and Vials as well as counting and analytical – solutions that support you throughout the process.

• Dissociation Reagents:

A broad range of Tissue Dissociation Reagents – the right one for most applications See Dissociation Reagents Dissociation Reagents.

• Organoid Formation and Cultivation:

CERO 3D incubator & Analyzer is a novel solution, allowing even long-term expansion of spheroids and organoids in 3D suspension without shear forces and in most cases without requirement for a supporting matrix. See CERO 3D incubator & Analyzer.