Explore the brand new HiMedia portfolio of animal cell culture as HiMedia distributor for Germany, Austria and Switzerland.
HiMedia offers a broad range of classical media, balanced salt solutions, reagents, sera, consumables and plasticware and chemicals and biochemicals required for cell culture. They try to meet the evolving needs of biologists by consistently upgrading and increasing our product profile. They cater to all the needs of cell culture for research and industrial purposes globally.
Cell Culture Media
Cell Culture Media Overview
Classical-, Insect-, HiGlutaXL- and LoSera- Media
The product portfolio includes several hundred of cell culture media. This includes different variations of classical media and as well as special media. Depending on the product, bottles volume for liquid media includes 50ml, 100ml, 500ml, 1000ml while packaging for powder media range from 1L to 100L.
For detailed information about Media download the catalog here:
If you cannot find the media you need, customization is our strength!
Contact us and lets us know your requirements whether it is different kind of packaging, variations in the combination of salts or buffers, or other requirements. We will try our best to support you with the solution you need, so you can focus on your work, instead of bothering about preparing media.
HiSepTM – Lymphocyte Separation Media
PBMCs – Peripheral Blood mononuclear cells – are a mixture of many subsets of lymphocytes and monocytes. The isolation of PBMCs is fundamental for immunology research.
HiSep™ Lymphocyte Separation Media are sterile, ready to use, density gradient media for isolating viable mononuclear cells from human blood and other animal species. They are made up of a polymer of sucrose with high synthetic molecular weight. Mononuclear cells (lymphocytes and monocytes) and platelets are contained in the banded plasma-LSM interphase due to their density and the pellet that is formed contains mostly erythrocytes and granulocytes, which have migrated through the gradient to the bottom of the tube.
- Highest consistency of different lots of LS001 with respect to purity of isolated PBMC’s
- Very low Endotoxin level <0.3 EU/ml
- Manufactured in GMP, ISO 13485 and ISO 9001 certified facility
- CE Marked for IVD(98/79/EC)
- Performance Test 95 ± 5% mononuclear cells
- 5 ± 2% red blood cells
- 3 ± 2% granulocytes
- >90% viability of separated cells
| Code | Product Name | Density (g/ml) | Cell type separated |
|---|---|---|---|
| LS001 | HiSep™ LSM 1077 | 1.077 | Human mononuclear cells (Lymphocytes and monocytes) |
| LS002 | HiSep™ LSM 1073 | 1.073 | Lower density human mononuclear cells (eg- mesenchymal stromal cells or monocytes). |
| LS003 | HiSep™ LSM 1084 | 1.084 | Mononuclear cells of higher density from Chicken, Rats, Mice and other Mammals) |
| LS004 | GranuloSep™ GSM1119 | 1.119 | Human mononuclear cells and granulocytes |
| LS005 | Ficoll 400-20% | Commonly used to prepare density gradients | |
| LS006 | HiSep™ LSM 1077 with 10mM HEPES | 1.077 | Human mononuclear cells (Lymphocytes and monocytes) |
Purity and viability are two major criteria for the performance of Separation media. Hence, both are controlled very strictly for each batch including use of Flow Cytometry and CASY counting and volume-based size detection.
Flow cytometric analysis showing lymphocytes and monocytes gated populations based on forward scatter (FSC) and side scatter (SSC).
PBMC sample analyzed using CASY cell counter & analyzer showing
- lymphocytes and monocytes gated population based on their volume
- Cell number and viability and cell counts
For more information about CASY click here
Guide - HiSepTM Lymphocyte Separation Medium
Explore all available solutions for separation of mononuclear cells
LS001 - HiSep™ LSM 1077
Isolation of mononuclear Cell from peripheral human blood
LS002 - HiSep™ LSM 1073
Isolation of lower density human mononuclear cells (e.g. mesenchymal stem cells or monocytes)
LS003 - HiSep™ LSM 1084
Isolation of Mononuclear cells of higher density from Chicken, Rats, Mice and other mammals)
LS004 - GranuloSep™ GSM 1119
Isolation of human mononuclear cells and granulocytes
LS005 - Ficoll 400-20%
Commonly used to prepare density gradients
LS006 - HiSep™ LSM 1077 with 10mM HEPES
Isolation of Mononuclear Cell from peripheral human blood
CryoXLTM – Cryopreservation
Cryopreservation is a central process in all cell culture laboratories. The right cryopreservation tools guarantee best recovery, viability and vitality after thawing. Below you will find a complete solution.
CryoXLTM Media enable highest viability of various kind of cell types. Solutions include:
- Cell Freezing Media – DMSO
- Cell Freezing Media – Glycerol
- Chemically Defined Serum Free Freezing Medium
- Stem Cell Freezing Medium
- Hybridoma Cell Freezing Medium
- Regular and Sensitive Cell Lines
CryoXL™ Cell Freezing Medium provides increased viability after thawing. These media contain either DMSO or Glycerol as a cryoprotective agent. TCL093 contains higher percentage of serum and has been optimized for freezing of sensitive cell lines such as insect cell lines, HEK293 and primary fibroblasts.
Post thaw recovery of different cell lines cryopreserved in CryoXL™ DMSO (product code: TCL043)
- Animal Origin Free and Serum-Free Formulation:
Developed to reduce regulatory hassles
Specially designed for cryopreservation of mammalian cells cultured in serum free conditions. Animal origin free formulation reduces the risk of contamination with adventitious agents.
Performance of CryoXL™ Serum Free Freezing Medium was evaluated using different serum free cultures and compared with commercially available serum free freezing media. Post-thaw recovery of cells cryopreserved in CryoXL™ Serum Free Freezing Medium was found to be at par with other commercially available media.
- Stem Cells – Improved post-thaw Recovery
Optimized to maintain differentiation potential
Cryopreservation of stem cells is quite difficult compared to standard cell types. These cells exhibit higher rates of apoptosis and spontaneous differentiation after freezing and thawing. With CryoXL™ medium for stem cells more than 85% of the original cell population can be successfully recovered without affecting their functional properties such ad differentiation potential and surface marker expression.
Performance of CryoXL™ Stem Cell Freezing Medium was evaluated using Human Wharton’s Jelly -(HWJ-MSC), Human Amnion Derived- (HAD-MSC) Mesenchymal Stem Cells, Human Dental Pulp-Stem Cells (HDP-SC) and compared with the traditional cryopreservation medium. CryoXL™ Stem Cell Freezing Medium exhibits higher post thaw recovery compared to the traditional medium.
- Developed for sensitive Hybridoma cells
Preservation without affecting biochemical functions
This medium is optimized for cryopreservation of Hybridoma cell lines without affecting their antibody productivity.
Guide Cryopreservation
Explore best solution for freezing and thawing
TCL043 CryoXL Cell Freezing Medium-DMSO, 1X
For regular cells, contains FBS and DMSO
TCL093 CryoXL Cell Freezing Medium-DMSO, 1X
Contains high concentration of FBS and can be used for sensitive cells
TCL056 CryoXL Cell Freezing Medium-DMSO, 1X , without Phenol Red
For hardy cell lines that are less susceptible to freezing damage
TCL044 CryoXL Cell Freezing Medium-Glycerol, 1X
Contains FBS and 10% Glycerol for hardy cell lines that are less susceptible to freezing damage
TCL078 CryoXL Cell Freezing Medium-Glycerol, 1X, without Phenol Red
Contains FBS and 10% Glycerol for hardy cell lines that are less susceptible to freezing damage
TCL098 FREEZin1
Serum free cell freezing medium for cells grown in serum free and animal component free conditions
TCL107 CryoXL™ Stem Cell Freezing Medium
Contains DMSO and FBS for human stem cells
Contact us
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28359 Bremen, Germany
