Cell Culture Media | Cultivation - Separation - Storage

Explore the brand new HiMedia portfolio of animal cell culture as HiMedia distributor for Germany, Austria and Switzerland.

HiMedia offers a broad range of classical media, balanced salt solutions, reagents, sera, consumables and plasticware and chemicals and biochemicals required for cell culture. They try to meet the evolving needs of biologists by consistently upgrading and increasing our product profile. They cater to all the needs of cell culture for research and industrial purposes globally.

An Overview

Classical-, Insect-, HiGlutaXL- and LoSera- Media

The product portfolio includes several hundred of cell culture media. This includes different variations of classical media and as well as special media. Depending on the product, bottles volume for liquid media includes 50ml, 100ml, 500ml, 1000ml while packaging for powder media range from 1L to 100L.

For detailed information about Media download the catalog here:

HiMedia Catalog

Explore the whole portfolio

Price List

Download the current price liste

If you cannot find the media you need, customization is our strength!

Contact us and lets us know your requirements whether it is different kind of packaging, variations in the combination of salts or buffers, or other requirements. We will try our best to support you with the solution you need, so you can focus on your work, instead of bothering about preparing media.

HiSep^TM – Lymphocyte Separation Media

PBMCs – Peripheral Blood mononuclear cells – are a mixture of many subsets of lymphocytes and monocytes. The isolation of PBMCs is fundamental for immunology research.

HiSep™ Lymphocyte Separation Media are sterile, ready to use, density gradient media for isolating viable mononuclear cells from human blood and other animal species. They are made up of a polymer of sucrose with high synthetic molecular weight. Mononuclear cells (lymphocytes and monocytes) and platelets are contained in the banded plasma-LSM interphase due to their density and the pellet that is formed contains mostly erythrocytes and granulocytes, which have migrated through the gradient to the bottom of the tube.

Highest consistency of different lots of LS001 with respect to purity of isolated PBMC’s
Very low Endotoxin level <0.3 EU/ml
Manufactured in GMP, ISO 13485 and ISO 9001 certified facility
CE Marked for IVD(98/79/EC)
Performance Test 95 ± 5% mononuclear cells
5 ± 2% red blood cells
3 ± 2% granulocytes
>90% viability of separated cells

Code	Product Name	Density (g/ml)	Cell type separated
LS001	HiSep™ LSM 1077	1.077	Human mononuclear cells (Lymphocytes and monocytes)
LS002	HiSep™ LSM 1073	1.073	Lower density human mononuclear cells (eg- mesenchymal stromal cells or monocytes).
LS003	HiSep™ LSM 1084	1.084	Mononuclear cells of higher density from Chicken, Rats, Mice and other Mammals)
LS004	GranuloSep™ GSM1119	1.119	Human mononuclear cells and granulocytes
LS005	Ficoll 400-20%		Commonly used to prepare density gradients
LS006	HiSep™ LSM 1077 with 10mM HEPES	1.077	Human mononuclear cells (Lymphocytes and monocytes)

Purity and viability are two major criteria for the performance of Separation media. Hence, both are controlled very strictly for each batch including use of Flow Cytometry and CASY counting and volume-based size detection.

Flow cytometric analysis showing lymphocytes and monocytes gated populations based on forward scatter (FSC) and side scatter (SSC).

PBMC sample analyzed using CASY cell counter & analyzer showing

lymphocytes and monocytes gated population based on their volume
Cell number and viability and cell counts

Get more information about CASY Cell Counter and Analyzer

Downloads - HiSep^TM

Guide - HiSepTM Lymphocyte Separation Medium

Explore all available solutions for separation of mononuclear cells

LS001 - HiSep™ LSM 1077

Isolation of mononuclear Cell from peripheral human blood

LS002 - HiSep™ LSM 1073

Isolation of lower density human mononuclear cells (e.g. mesenchymal stem cells or monocytes)

LS003 - HiSep™ LSM 1084

Isolation of Mononuclear cells of higher density from Chicken, Rats, Mice and other mammals)

LS004 - GranuloSep™ GSM 1119

Isolation of human mononuclear cells and granulocytes

LS005 - Ficoll 400-20%

Commonly used to prepare density gradients

LS006 - HiSep™ LSM 1077 with 10mM HEPES

Isolation of Mononuclear Cell from peripheral human blood

CryoXL^TM – Cryopreservation

Cryopreservation is a central process in all cell culture laboratories. The right cryopreservation tools guarantee best recovery, viability and vitality after thawing. Below you will find a complete solution.

CryoXL^TM Media enable highest viability of various kind of cell types. Solutions include:

Cell Freezing Media – DMSO
Cell Freezing Media – Glycerol
Chemically Defined Serum Free Freezing Medium
Stem Cell Freezing Medium
Hybridoma Cell Freezing Medium

Regular and Sensitive Cell Lines

CryoXL™ Cell Freezing Medium provides increased viability after thawing. These media contain either DMSO or Glycerol as a cryoprotective agent. TCL093 contains higher percentage of serum and has been optimized for freezing of sensitive cell lines such as insect cell lines, HEK293 and primary fibroblasts.

Post thaw recovery of different cell lines cryopreserved in CryoXL™ DMSO (product code: TCL043)

Animal Origin Free and Serum-Free Formulation:

Developed to reduce regulatory hassles

Specially designed for cryopreservation of mammalian cells cultured in serum free conditions. Animal origin free formulation reduces the risk of contamination with adventitious agents.

Performance of CryoXL™ Serum Free Freezing Medium was evaluated using different serum free cultures and compared with commercially available serum free freezing media. Post-thaw recovery of cells cryopreserved in CryoXL™ Serum Free Freezing Medium was found to be at par with other commercially available media.

Stem Cells – Improved post-thaw Recovery

Optimized to maintain differentiation potential

Cryopreservation of stem cells is quite difficult compared to standard cell types. These cells exhibit higher rates of apoptosis and spontaneous differentiation after freezing and thawing. With CryoXL™ medium for stem cells more than 85% of the original cell population can be successfully recovered without affecting their functional properties such ad differentiation potential and surface marker expression.

Performance of CryoXL™ Stem Cell Freezing Medium was evaluated using Human Wharton’s Jelly -(HWJ-MSC), Human Amnion Derived- (HAD-MSC) Mesenchymal Stem Cells, Human Dental Pulp-Stem Cells (HDP-SC) and compared with the traditional cryopreservation medium. CryoXL™ Stem Cell Freezing Medium exhibits higher post thaw recovery compared to the traditional medium.