CryoXLTM – Cryopreservation

Cryopreservation is a central process in all cell culture laboratories. The right cryopreservation tools guarantee best recovery, viability and vitality after thawing. The HiMedia catalog encompasses complete solutions for any freezing workflow – from cryomedia to cryovials.

Increased viability after thawing – no matter if the cell line is sensitive or regular:

CryoXL™ Cell Freezing Medium contains either DMSO or Glycerol as a cryoprotective agent. TCL093 contains higher percentage of serum and has been optimized for freezing of sensitive cell lines such as insect cell lines, HEK293 and primary fibroblasts.

Post thaw recovery of different cell lines cryopreserved in CryoXL™ DMSO (product code: TCL043)

Animal Origin Free and Serum-Free Formulation – Developed to reduce regulatory hassle:

Specially designed for cryopreservation of mammalian cells cultured in serum free conditions. Animal origin free formulation reduces the risk of contamination with adventitious agents.

Performance of CryoXL™ Serum Free Freezing Medium was evaluated using different serum free cultures and compared with commercially available serum free freezing media. Post-thaw recovery of cells cryopreserved in CryoXL™ Serum Free Freezing Medium was found to be at par with other commercially available media.

Stem Cells – Improved post-thaw recovery – Optimized to maintain differentiation potential:

Cryopreservation of stem cells is quite difficult compared to standard cell types. These cells exhibit higher rates of apoptosis and spontaneous differentiation after freezing and thawing. With CryoXL™ medium for stem cells more than 85% of the original cell population can be successfully recovered without affecting their functional properties such ad differentiation potential and surface marker expression.

Performance of CryoXL™ Stem Cell Freezing Medium was evaluated using Human Wharton’s Jelly -(HWJ-MSC), Human Amnion Derived- (HAD-MSC) Mesenchymal Stem Cells, Human Dental Pulp-Stem Cells (HDP-SC) and compared with the traditional cryopreservation medium. CryoXL™ Stem Cell Freezing Medium exhibits higher post thaw recovery compared to the traditional medium.

In adipose derived mesenchymal stem cells before freezing and after thawing.

Developed for sensitive Hybridoma cells – Preservation without affecting biochemical functions:

This medium is optimized for cryopreservation of Hybridoma cell lines without affecting their antibody productivity.