HiSep™
Lymphocyte Separation Media

PBMCs – Peripheral Blood mononuclear cells – are a mixture of many subsets of lymphocytes and monocytes. The isolation of PBMCs is fundamental for immunology research.
HiSep™ Lymphocyte Separation Media are sterile, ready to use, density gradient media for isolating viable mononuclear cells from human blood and other animal species. They are made up of a polymer of sucrose with high synthetic molecular weight. Mononuclear cells (lymphocytes and monocytes) and platelets are contained in the banded plasma-LSM interphase due to their density and the pellet that is formed contains mostly erythrocytes and granulocytes, which have migrated through the gradient to the bottom of the tube.

• Highest consistency of different lots of LS001 with respect to purity of isolated PBMC’s
• Very low Endotoxin level <0.3 EU/ml
• Manufactured in GMP, ISO 13485 and ISO 9001 certified facility
• CE Marked for IVD(98/79/EC)
• Performance Test 95 ± 5% mononuclear cells
• 5 ± 2% red blood cells
• 3 ± 2% granulocytes
• >90% viability of separated cells

Purity and viability are two major criteria for the performance of Separation media. Hence, both are controlled very strictly for each batch including use of Flow Cytometry and CASY counting and volume-based size detection.

Flow cytometric analysis showing lymphocytes and monocytes gated populations based on forward scatter (FSC) and side scatter (SSC).

PBMC sample analyzed using CASY cell counter & analyzer showing

• lymphocytes and monocytes gated population based on their volume
• Cell number and viability and cell counts

Get more information about CASY Cell Counter and Analyzer