Real-Time Live-Cell Imaging System xCELLigence® RTCA eSight

Quantitative Data and Image Analysis in an Incubator

Dual Mode – Incubator Microscope – Multiple Assays

Key Features of Analysis and Live Cell Imaging xCELLigence® RTCA eSight

Real Time Live Cell Imaging System xCELLigence® RTCA eSight

  • Combines real time cell analysis (impedance) and live cell imaging simultaneously
  • Equipped with 3 fluorescence channels (red, green, blue) + brightfield
  • Availability of 5 cradles (3 for impedance + imaging; 2 for imaging only)
  • Measures impedance 96 well-plate in 15 seconds; images a 96 well-plate in 6 minutes
  • Intuitive data analysis RTCA software

Analyze and Image your cells in Real Time

xCELLigence® Real Time Cell Analyzer (RTCA) eSight is like an incubator microscope. It allows the analysis and live cell imaging of your cell culture in real time. Like the live cell analysis xCELLigence® RTCA, the xCELLigence® eSight also works on the principle of impedance

The xCELLigence® RTCA eSight is the first its kind, a Dual Function Cell Analyzer.

Simple workflow: It has a simple workflow with easy acquisition & in-depth analysis and can be used to obtain more information/data.

One experiment, two perspectives: As an incubator microscope, the real time live cell imaging system xCELLigence® RTCA eSight gives you 2 perspectives – the imaging data and the accurate, real time kinetics measured by biosensors on the same cell populations, independently or simultaneously.

Quantitative data about cell behavior in real time: Acquire details into cell quality and function since the xCELLigence® RTCA eSight provides unprecedented level of details into cells for quality assessment such as cell number, size, and adhesion, without any user intervention to generate physiologically relevant results.

Wide range of assays: It can be used in a wide range of cell-based assays such as proliferation, cytotoxicity and apoptosis. Track cell behavior and generate quantitative real time adhesion and morphological data and learn about mechanism of actions using imaging coupled with the time resolution of live tracking functionality.

Confidence in the data: There is an additional value of collecting data from a single plate on the same cell populations and gain insightful information on cell behavior. Running two assays from the same cell populations provides an internal control and less variability than working with multiple replicate plates. Since one can run parallel assays in the same plate, in the same instrument, and on the same cells, one can eliminate any doubt of inconsistency created by plate-to-plate, material-to-material, and instrument-to-instrument variability.

Multiplexing: The power of multiplexing offers information “richness”. The eSight instrument allows the tracking of cell health and behavior simultaneously, and of the same cell populations, from the very different vantage points of real-time cellular impedance and live cell imaging.

Tracking Apoptosis with xCELLigence® RTCA eSight

RTCA eSight software for impedance and imaging data

The RTCA eSight software has a user-friendly and intuitive interface. It makes setting up of experiments and data analysis easy for the users. The users can set-up and define 5 different assays at a time with defined schedules.

There is no need to stop any assay anymore! All the 5 assays can be run parallely and independently!!

For the Live-Cell analysis and Imaging, the RTCA eSight software follows a simple workflow to batch process huge image sets.

The Workflow for image analysis

We‘ve searched an alternative to CRA, ideally without the need for using dyes or being limited by endpoint-assays. xCELLigence turned out to be the most suitable and easy to install. We now achieve an effector to target ratio of 0.05:1, evaluate complete kinetics and even might use effector cells in further experiments.

J. Peper, IFIZ Tübingen, Abt. Immunologie, Germany

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