The fastest way to a single cell
The Namocell Single Cell Dispensers make single cell isolation and sorting effortless. These benchtop devices use proprietary microfluidics technology to sort and dispense single cells directly into 96-well or 384-well plate. It simplifies and empowers a number of single cell applications, including cell line development, single cell genomics, monoclonal antibody development, CRISPR selection, synthetic biology, circulating tumor cells (CTCs) isolation and circulating fetal cells isolation. It is affordable, user-friendly, and requires almost no training to operate. Namocell has combined the benefits of three key technologies: flow cytometry, microfluidics and liquid dispensing. The combination of these techniques is unique and enables users to accomplish single cell sorting and dispensing in one step, providing fast, efficient and gentle isolation of single cells. The Pala™ and Hana™ Single Cell Dispensers are capable of isolating single cells in a high throughput manner that is still gentle on cells so that they can maintain their viability. Namocell’s microfluidic cell cartridges ensure that there is no sample-to-sample cross contamination and keep the sample sterile. Cartridges that contain the sample material can be swapped out so there is no need for cleaning process.
Single Cell Genomics
Advances in single cell genomics have revolutionized the study of cellular heterogeneity. The body’s composition trillions of cells offer individual distinct molecular profiles to be investigated. Advancements in tools such as RNA sequencing of single cells (scRNA-seq) has been used to identify and define the variety of cell subtypes. The Namocell Single Cell Dispenser provides a simple, affordable, and flexible platform for any single cell study. The ability to sort and isolate only live cells or a sub-population of cells provide new opportunities for single cell studies.
Cell Line Development and monoclonal antibodies
The CHO cell line has been widely used for the production of therapeutic proteins. In a typical cell line development workflow, CHO cells are transfected with an IgG expressing vector. A single CHO cell is obtained by limited dilution cloning. A high titer clone is selected using ELISA. Limiting dilution is unreliable and many wells are either empty or have multiple cells. This process of selecting high titer clones is extremely inefficient and costly. The Namocell Single Cell Dispenser is able to dispense single cells into 90% of the wells. Using fluorescence sorting capabilities, Namocell Single Cell Dispenser is able to not only isolate single cells but also isolate single cells that have high antibody production.
A powerful gene-editing technology, CRISPR allows for easy and quick ways to modify any genes in a given cell type. In a typical CRISPR workflow the cells are transfected with CRISPR constructs. A fluorescent-labeled marker can also be co-transfected. Once the cells have recovered, a single cell is isolated using limited dilution. The isolated single cells are allowed to grow. The knockout clones are identified with PCR. Limiting dilution is unreliable and many wells are either empty or have multiple cells. When high throughput CRISPR workflow is required, limited dilution becomes extremely inefficient and costly. Namocell Single Cell Dispenser is able to dispense single cells into 90% of the wells. Using fluorescence sorting capabilities, Namocell Single Cell Dispenser is able to not only isolate single cells but also isolate knock-in or knockout cells when the gene is tagged with fluorescent proteins.
Rare Cell Analysis
Rare cells are extremely low-abundance cells within a much larger population of background cells. These rare cells such as circulating tumor cells and fetal cells provide early detection and analytical information that could replace invasive diagnostic procedures. The challenge of isolating these rare cells is due to their extremely low availability. Therefore, it is essential to have high efficiency and sensitivity to isolate these cells. Based on the positive fluorescent labeled markers of rare cells, the Namocell Single Cell Dispenser uses a combination of microfluidics, flow cytometry, and liquid dispensing to isolate one out of a million cells quickly and efficiently. Isolated rare cells can be used for cell culture or genomic analysis.
|Namocell Single Cell Dispenser||Traditional Sorter|
|Pressure||Less than 2 psi||20 - 70 psi|
|Initialization time||2 minutes||45 minutes|
|Processing speed||2 - 4.000 cells/s||2.000 - 20.000 cells/s|
|Channels||2 - 11||16|
|Droplet size||1 µl||0,001 - 0,005 µl|
|Sheath||15 mL/hr||1-2 L/hr|
Dr. Thorsten Rieling
Webinar | On Demand Webinar | 10 am CEST
Whether you’re a beginner or expert in single-cell analysis, join this informative webinar to learn about the considerations, challenges and new technologies for low-input or rare single-cell genomics, followed by a discussion on the latest tips for low-input NGS. Speakers: Felix Alonso-Valenteen Ph.D. (Field Application Scientist Namocell) Ioanna Andreou, Ph.D. (Associate Director R&D, NGS Applications Development QIAGEN)Read more ›