Understanding the complexity and key challenges involved in vaccine production process, a chemically defined, serum-free, animal component free medium – such as CELLin1™ – fulfills an essential role. This medium is suitable for virus cultivation in broad range of cell lines such as Vero, MDCK, MDBK, PK-15 and MRC-5.
Banking of host cell line and preparation of the seed are the two major preliminary steps involved in viral vaccine production. CELLin1™ supports continuous growth of host cells through multiple subcultures, making it suitable for banking. Consistent cell growth over serial subcultures, suitable for cell expansion and banking.
Vero, PK-15, MDCK and MDBK cells were seeded in CELLin1™ in T175 flask. Consistent cell density was obtained throughout 5 subcultures and viability of each cell was more than 90%, making it suitable for host cell expansion and banking.
Cells are grown in classical 2D monolayer format during cell banking and expansion phase whereas, 3D microcarrier culturing is performed during actual virus cultivation and vaccine production phase. CELLin1™ has been optimized to support the growth of cells in 2D as well as 3D microcarrier system. Consistent cell growth over serial subcultures, suitable for cell expansion and banking.
Cultivation period for most viruses in cell line host ranges from 2-4 days. CELLin1™ supports sustained growth of host cells high cell densities on harvest, thereby assuring optimum virus multiplication.
Vero cells were seeded in CELLin1™ and competitor medium at 0.05*106 in T175 flasks. Cells were cultured at 37°C, 5% CO2, for 4 days. At each point in time, the cell viability in CELLin1™ was more than 90% throughout 4 days.
[SFM036L] Price on request
A chemically defined, animal component-free medium designed for growth and maintenance of Vero, MDCK, MDBK, PK15 and MRC-5 cells. The medium is devoid of L-Glutamine.
All the HEK-based vaccine manufacturing processes require a highly efficient and productive serum free and animal component free medium at each step starting from cell banking to cell expansion and virus production in bioreactor. HEKin1™ is a serum free and animal component free medium optimized for the growth and expansion of HEK293 cells under serum-free conditions. This is a complete media that will support growth of HEK293 cells without further supplementation. This media has been tested for its ability to support high-density cultures of HEK293 cells.
Price on request
HEK-in-1 is a complete, ready-to-use medium for the cultivation of HEK cells.
Chemically defined, animal component-free, serum-free virus production medium
Serum-free and animal component-free medium designed for HEK293 cells
Dr. Sahar Rafizadeh