Dissociation – Cell Culture – Cell Isolation – Assay – Imaging & Analysis
Stem cells are undifferentiated cells that have the remarkable ability to differentiate into various specialized cell types. Stem cell in vitro models offer a great source for a better insight into how diseases occur and for developing new medical treatments. This gives hope for the future that they might be able to repair damaged tissues or diseased organs.
Stem cells can be broadly categorized into several types such as embryonic stem cells (ESCs), adult stem cells and induced pluripotent stem cells (iPSCs).
Embryonic Stem Cells (ESC) derived from early-stage embryos possess the broadest differentiation potential, meaning they can become any cell type in the body and can be used as in vitro models. However, the use of ESCs in the lab raises ethical concerns as embryonic cells from early stage (blastocytes) are needed.
Adult Stem Cells can be found in low numbers in tissues of the body. Their differentiation potential is limited compared to ESCs. However, they still offer significant therapeutic potential. While adult stem cells must be extracted from, partly hard to access, tissues, there is another stem cell type, called induced pluripotent stem cells (iPSC) that can be reprogrammed from cells derived from easily accessible tissues, such as blood or epithelial cells that can be found in urine.
Induced pluripotent stem cells (iPSC) bring further value to the research. These are reprogrammed somatic cells. They are reprogrammed by introducing suitable transcription factors into the cells whose over-expression induces pluripotency in these cells.
Since they are reprogrammed from cells isolated from adult tissue, such as skin, blood and diverse other tissues, they raise no ethical concerns. iPSCs have almost the same differentiation potential as ESCs, making them indispensable for future medicine.
For stem cell models, reliable workflows are needed. Here you find an overview of a workflow starting with tissue dissociation and cell culture, following single cell isolation and ending with analysis and imaging.
TIGR Tissue Grinder & Dissociator is an enzyme-free approach for generating single cells from tissue.TIGR Tissue Grinder
CASY Cell Counter & Analyzer allows reliable counting of different cell types, as well as fast viability analyses.CASY Cell Counter
CERO 3D Incubator & Bioreactor ensures automated cultivation of stem cells, spheroids, organoids and even tissues.CERO 3D
The CellRaft AIR System is a better way to handle sensitive cells in three steps: Image – Identify – IsolateCellRaft AIR
CEROplates with ultra-low attachment offer a simplified process for growing 3D aggregates.CEROplates