Stem cells – Spheroids – Organoids – Tissues
Think in new dimensions – CERO CD culture incubator is overcoming known limitations of static cultures.
Easy, ready-to-use protocols, standardizable workflows and autoadhesion of cells without any need for Carriers or Matrigel are just a few of the advantages.
As a result, CERO enables high yield expansion of pluripotent stem cells or long term cultivation of tissue for >20 days (Spheroids >80 or Organoids >180).
Are you interested in our CERO 3D Application Handbook? Feel free to download it and contact us. We would be happy to get in touch with you.
Our group is focusing on primary tumor cells grown on 3D polymer scaffolds. For that we are using CERO 3D to develop a model of drug development and precision medicine. It is particularly important that the cells are viable and proliferating in 3D complex structures as seen in tumor tissues. With the CERO 3D we are implementing and developing tools for patient-specific tumor therapy. Furthermore, the cancer cells isolated from fresh tumor biopsies make treatment optimization possible before a therapy is initiated. Here, CERO 3D incubator & bioreactor enables us to develop personalized medicine in a 3D cell culture model.
Cultivating hepatocyte spheroids in CERO considerably improves expansion, differentiation, maturation and hepatic virus infection compared to monolayer culture. Our research takes advantage from healthy cells even from long-term cultures in CERO. Moreover, we are now able to perform 3D long-term culture of human tissue specimen in CERO - a paradigm shift.
Generation of HepaRG Spheroids in the CERO
Scientific Poster: "Establishment and validation of the 3D cultivation system“CERO” for hepatic spheroids to study viral hepatitis", presented at the 2018 EMBL Symposium "Organoids: Modelling Organ Development and Disease in 3D Culture"
Expansion Protocol Pluripotent Stem Cells (iPSC and ESC)
Differentiation Protocol Organoids from Pluripotent Stem Cells
Instructions for setting system out of Operation, no use for > 4 weeks